Correction:A study of the structural properties of sites modified by the O-linked 6-N-acetylglucosamine transferase

[This corrects the article DOI: 10.1371/journal.pone.0184405.]

RN7SK small nuclear RNA controls bidirectional transcription of highly expressed gene pairs in skin.

Funder: Wellcome TrustFunder: Medical Research CouncilPausing of RNA polymerase II (Pol II) close to promoters is a common regulatory step in RNA synthesis, and is coordinated by a ribonucleoprotein complex scaffolded by the noncoding RNA RN7SK. The function of RN7SK-regulated gene transcription in adult tissue homoeostasis is currently unknown. Here, we deplete RN7SK during mouse and human epidermal stem cell differentiation. Unexpectedly, loss of this small nuclear RNA specifically reduces transcription of numerous cell cycle regulators leading to cell cycle exit and differentiation. Mechanistically, we show that RN7SK is required for efficient transcription of highly expressed gene pairs with bidirectional promoters, which in the epidermis co-regulated cell cycle and chromosome organization. The reduction in transcription involves impaired splicing and RNA decay, but occurs in the absence of chromatin remodelling at promoters and putative enhancers. Thus, RN7SK is directly required for efficient Pol II transcription of highly transcribed bidirectional gene pairs, and thereby exerts tissue-specific functions, such as maintaining a cycling cell population in the epidermis

Erratum to: The study of cardiovascular risk in adolescents – ERICA: rationale, design and sample characteristics of a national survey examining cardiovascular risk factor profile in Brazilian adolescents

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Appendix 1 - A study of the structural properties of sites modified by the O-linked 6-N-acetylglucosamine transferase

Sites' properties for SS132 dataset. List of all entries in the SS132 dataset. PDB id, PDB identifier; Chain, PDB chain; Position, residue position within the chain; Cluster id, cluster id in Figure 4. B-factor, site mean C-alpha chain standardised B-factor, RSA, site average relative solvent accessibility; SS, DSSP secondary structure assignments